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Promega pcineo mammalian expression vector
Pcineo Mammalian Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcineo+mammalian+expression+vector/pcineo+vector/pmc02978134-160-6-10
Average 90 stars, based on 1 article reviews
pcineo mammalian expression vector - by Bioz Stars, 2026-10
90/100 stars

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Clone Assay:

Article Title: Covalent diabodies and uses thereof
Article Snippet: PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2. .. Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2. .. Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: The forward primers of the VL domains (i.e., SEQ ID NO:57) and reverse primers of the VH domains (i.e., SEQ ID NO:60 and SEQ ID NO:63) contained unique restriction sites to allow cloning of the final product into an expression vector. .. Purified overlapping PCR products were digested with restriction endonucleases Nhe I and EcoR I, and cloned into the pCIneo mammalian expression vector (Promega, Inc.). .. The plasmids encoding constructs were designated as identified in Table 12: TABLE 12 PLASMID CONSTRUCTS Encoding Construct Plasmid Designation Insert 1 pMGX0669 hu2B6VL-hu3G8VH 2 pMGX0667 hu3G8VL-hu2B6VH 3 pMGX0666 hu3G8VL-hu3G8VH 4 pMGX0668 hu2B6VL-hu2B6VH Polypeptide/Diabody Expression: pMGX0669, encoding construct 1, was cotransfected with pMGX0667, encoding construct 2, in HEK-293 cells using Lipofectamine 2000 according to the manufacturer's directions (Invitrogen).

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Expressing:

Article Title: Covalent diabodies and uses thereof
Article Snippet: PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2. .. Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Identification and structural analysis of C-terminally truncated collapsin response mediator protein-2 in a murine model of prion diseases
Article Snippet: .. The cDNAs were ligated into the pCIneo mammalian expression vector (Promega) between the Eco RI and Not I sites. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2. .. Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Identification of JNK1 as a predicting biomarker for ABT-199 and paclitaxel combination treatment.
Article Snippet: Targeting Bcl-2 with ABT-199 (Venetoclax) shows limited single-agent activity against many cancers in both preclinical and clinical investigations.. Combination therapies have attracted great attention.. The principal purpose of this study was to investigate the mechanism of synergism between ABT-199 and paclitaxel.

Article Title: Covalent diabodies and uses thereof
Article Snippet: The forward primers of the VL domains (i.e., SEQ ID NO:57) and reverse primers of the VH domains (i.e., SEQ ID NO:60 and SEQ ID NO:63) contained unique restriction sites to allow cloning of the final product into an expression vector. .. Purified overlapping PCR products were digested with restriction endonucleases Nhe I and EcoR I, and cloned into the pCIneo mammalian expression vector (Promega, Inc.). .. The plasmids encoding constructs were designated as identified in Table 12: TABLE 12 PLASMID CONSTRUCTS Encoding Construct Plasmid Designation Insert 1 pMGX0669 hu2B6VL-hu3G8VH 2 pMGX0667 hu3G8VL-hu2B6VH 3 pMGX0666 hu3G8VL-hu3G8VH 4 pMGX0668 hu2B6VL-hu2B6VH Polypeptide/Diabody Expression: pMGX0669, encoding construct 1, was cotransfected with pMGX0667, encoding construct 2, in HEK-293 cells using Lipofectamine 2000 according to the manufacturer's directions (Invitrogen).

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Plasmid Preparation:

Article Title: Covalent diabodies and uses thereof
Article Snippet: PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2. .. Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Identification and structural analysis of C-terminally truncated collapsin response mediator protein-2 in a murine model of prion diseases
Article Snippet: .. The cDNAs were ligated into the pCIneo mammalian expression vector (Promega) between the Eco RI and Not I sites. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2. .. Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Identification of JNK1 as a predicting biomarker for ABT-199 and paclitaxel combination treatment.
Article Snippet: Targeting Bcl-2 with ABT-199 (Venetoclax) shows limited single-agent activity against many cancers in both preclinical and clinical investigations.. Combination therapies have attracted great attention.. The principal purpose of this study was to investigate the mechanism of synergism between ABT-199 and paclitaxel.

Article Title: Covalent diabodies and uses thereof
Article Snippet: The forward primers of the VL domains (i.e., SEQ ID NO:57) and reverse primers of the VH domains (i.e., SEQ ID NO:60 and SEQ ID NO:63) contained unique restriction sites to allow cloning of the final product into an expression vector. .. Purified overlapping PCR products were digested with restriction endonucleases Nhe I and EcoR I, and cloned into the pCIneo mammalian expression vector (Promega, Inc.). .. The plasmids encoding constructs were designated as identified in Table 12: TABLE 12 PLASMID CONSTRUCTS Encoding Construct Plasmid Designation Insert 1 pMGX0669 hu2B6VL-hu3G8VH 2 pMGX0667 hu3G8VL-hu2B6VH 3 pMGX0666 hu3G8VL-hu3G8VH 4 pMGX0668 hu2B6VL-hu2B6VH Polypeptide/Diabody Expression: pMGX0669, encoding construct 1, was cotransfected with pMGX0667, encoding construct 2, in HEK-293 cells using Lipofectamine 2000 according to the manufacturer's directions (Invitrogen).

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Polymerase Chain Reaction:

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: The forward primers of the VL domains (i.e., SEQ ID NO:57) and reverse primers of the VH domains (i.e., SEQ ID NO:60 and SEQ ID NO:63) contained unique restriction sites to allow cloning of the final product into an expression vector. .. Purified overlapping PCR products were digested with restriction endonucleases Nhe I and EcoR I, and cloned into the pCIneo mammalian expression vector (Promega, Inc.). .. The plasmids encoding constructs were designated as identified in Table 12: TABLE 12 PLASMID CONSTRUCTS Encoding Construct Plasmid Designation Insert 1 pMGX0669 hu2B6VL-hu3G8VH 2 pMGX0667 hu3G8VL-hu2B6VH 3 pMGX0666 hu3G8VL-hu3G8VH 4 pMGX0668 hu2B6VL-hu2B6VH Polypeptide/Diabody Expression: pMGX0669, encoding construct 1, was cotransfected with pMGX0667, encoding construct 2, in HEK-293 cells using Lipofectamine 2000 according to the manufacturer's directions (Invitrogen).

Purification:

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: The forward primers of the VL domains (i.e., SEQ ID NO:57) and reverse primers of the VH domains (i.e., SEQ ID NO:60 and SEQ ID NO:63) contained unique restriction sites to allow cloning of the final product into an expression vector. .. Purified overlapping PCR products were digested with restriction endonucleases Nhe I and EcoR I, and cloned into the pCIneo mammalian expression vector (Promega, Inc.). .. The plasmids encoding constructs were designated as identified in Table 12: TABLE 12 PLASMID CONSTRUCTS Encoding Construct Plasmid Designation Insert 1 pMGX0669 hu2B6VL-hu3G8VH 2 pMGX0667 hu3G8VL-hu2B6VH 3 pMGX0666 hu3G8VL-hu3G8VH 4 pMGX0668 hu2B6VL-hu2B6VH Polypeptide/Diabody Expression: pMGX0669, encoding construct 1, was cotransfected with pMGX0667, encoding construct 2, in HEK-293 cells using Lipofectamine 2000 according to the manufacturer's directions (Invitrogen).

Construct:

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Amplification:

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..

Article Title: Covalent diabodies and uses thereof
Article Snippet: .. PCR and Expression Vector Construction: All PCR and PCR product purification protocols were as described in Example 6.1 and 6.2 Overlapping PCR: Final products were constructed, amplified and purified using methods described in example 6.1 and example 6.2 with appropriate primers Final products were cloned into pCIneo mammalian expression vector (Promega, Inc.) as previously described. ..



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